Composite

Part:BBa_K3718002:Design

Designed by: Chan Pui Ching   Group: iGEM21_Hong_Kong_UCCKE   (2021-10-02)


Customizable detection and expression with increased growth


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 67
    Illegal suffix found in sequence at 629
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 67
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 630
    Illegal PstI site found at 644
    Illegal NotI site found at 73
    Illegal NotI site found at 637
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 67
    Illegal BglII site found at 2337
    Illegal BamHI site found at 912
    Illegal XhoI site found at 906
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 67
    Illegal suffix found in sequence at 630
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 67
    Illegal XbaI site found at 82
    Illegal SpeI site found at 630
    Illegal PstI site found at 644
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

/


Source

/

References